	   SnapGene      cctgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttcctgcggccgcacgcgtgtgtctagactgcagagggccctgcgtatgagtgcaagtgggttttaggaccaggatgaggcggggtgggggtgcctacctgacgaccgaccccgacccactggacaagcacccaacccccattccccaaattgcgcatcccctatcagagagggggaggggaaacaggatgcggcgaggcgcgtgcgcactgccagcttcagcaccgcggacagtgccttcgcccccgcctggcggcgcgcgccaccgccgcctcagcactgaaggcgcgctgacgtcactcgccggtcccccgcaaactccccttcccggccaccttggtcgcgtccgcgccgccgccggcccagccggaccgcaccacgcgaggcgcgagataggggggcacgggcgcgaccatctgcgctgcggcgccggcgactcagcgctgcctcagtctgcggtgggcagcggaggagtcgtgtcgtgcctgagagcgcagtcgagaaggtaccggatcctctagagtcgactccggaataacttcgtataggatactttatacgaagttatgcagaatggtagctggattgtagctgctattagcaatatgaaacctcttaataacttcgtatagcatacattatacgaagttatggcgcgccctacttgtacagctcgtccatgccgagagtgatcccggcggcggtcacgaactccagcaggaccatgtgatcgcgcttctcgttggggtctttgctcagggcggactgggtgctcaggtagtggttgtcgggcagcagcacggggccgtcgccgatgggggtgttctgctggtagtggtcggcgagctgcacgctgccgtcctcgatgttgtggcggatcttgaagttcaccttgatgccgttcttctgcttgtcggccatgatatagacgttgtggctgttgtagttgtactccagcttgtgccccaggatgttgccgtcctccttgaagtcgatgcccttcagctcgatgcggttcaccagggtgtcgccctcgaacttcacctcggcgcgggtcttgtagttgccgtcgtccttgaagaagatggtgcgctcctggacgtagccttcgggcatggcggacttgaagaagtcgtgctgcttcatgtggtcggggtagcggctgaagcactgcacgccgtaggtcagggtggtcacgagggtgggccagggcacgggcagcttgccggtggtgcagatgaacttcagggtcagcttgccgtaggtggcatcgccctcgccctcgccggacacgctgaacttgtggccgtttacgtcgccgtccagctcgaccaggatgggcaccaccccggtgaacagctcctcgcccttgctcaccatggtggcgctagcataacttcgtataaagtatcctatacgaagttatttgccttaacccagaaattatcactgttattctttagaatggtgcaaagaataacttcgtataatgtatgctatacgaagttatgaattcgatatcaagcttatcgataatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgctcgcctatgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcatcgataccgagcgctgctcgagagatctacgggtggcatccctgtgacccctccccagtgcctctcctggccctggaagttgccactccagtgcccaccagccttgtcctaataaaattaagttgcatcattttgtctgactaggtgtccttctataatattatggggtggaggggggtggtatggagcaaggggcaagttgggaagacaacctgtagggcctgcggggtctattgggaaccaagctggagtgcagtggcacaatcttggctcactgcaatctccgcctcctgggttcaagcgattctcctgcctcagcctcccgagttgttgggattccaggcatgcatgaccaggctcagctaatttttgtttttttggtagagacggggtttcaccatattggccaggctggtctccaactcctaatctcaggtgatctacccaccttggcctcccaaattgctgggattacaggcgtgaaccactgctcccttccctgtccttctgattttgtaggtaaccacgtgcggaccgagcggccgcaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgcctgcaggggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatacgtcaaagcaaccatagtacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccttagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaactctatctcgggctattcttttgatttataagggattttgccgatttcggtctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaattttatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgt  1=   )         !               !            $         !                     	                              #               
                                 +         1               +         "   #      (                                                                                                                          
                                                                                    !                                    &   %                                             
               "   	                  5                           #                                 #               "                           !          3            #                                                /   	                  $   (            !                                  0   !         #           ) y    ().	
eo m	u	v	w			M
v[\TUVKLM b */:		

, z    f|{ ,\n	x			N
i}W! * 1  Is06<p		)CNw]KKN c +PS(r			
~oR 0			
[  7{E/

-[z/a9   aLw;c
$!   F}|!-HKl7IX	o	y	{
_~NR u|	D

d {  rk	O
jE&f{7U Xy < ;g$]	f
4=G$ + 2  =Bx{&,	58tD
*OxO
?O  J7=o<q		
8	_3h& $~	,
b
DB^L! " D h   10Ts		]


%RLn   `   &,@Q*&s	#$ ^ $dp)H
BlH d  T+)	

;  M Q U f	
[_cPhp0S rX	[		r| 19GWbM7\	 3?cj.n  AT	

 R3Pt  8F^mQ
l\${0@ ixTrJ/: 0	


p#.7UY7 |/U~
uAb  \<J
<D,  b	d=  /xS	2v%+ 5     Md'

-	ox# f   G"".Im.BGp	z		S K -S|


YR`>   Y}JSY	   Gf^~=L8L	#		
vxO] + i	E

+
 
fhe)  IP
}	
 v1= b(.I+5l%	B		


z8<! 
(^Fg3  U<
P
'|HuVZ | s	;kYz . = V[m[5!H4 <%^8L 0mG"kG  @hO		g
>  O?L2	[	
+   ;_y$DN69up8aq	

P6PEPl 3 O S > -	+]ah@Pr ,   EE
'21   W Y CI|~'5h
Y6egy
>?%@   [  KjI>N	
isuB  :=_			
.4Ai'7g prE	
#
9R`_   KZ8N	_+ 
 !7%Yx	c

lR\ozE0M TG2
Ij T[	9AbqE!Cxa3k"    ni	-

Q_NC# #  )&LEf{
&?4^ i P2w(	^
6}"c( E  ht:`t	-			 


So{MY@'QUo  & @  n?au{1U	%	8?J$f a x  '-rz+5[t			uh+JJ   6RKD'

 tkW	z

3%eM~  !  A;
+
a

$%> ] _  #%e*G
#kg q2>V-Lm Pkt@I  	I
Cu<n+ J e   e,	"			
Xuw 	e<g -	]*	A		

yt2 ?UlZF!  N R V 4Mg`	
\`d	ODq1  YH	Q-^f 	 S8h@ap	1
kPQ`ij ,	
eT s	2*I	}= =	jsjf*  Y\	@		ds  
o		OP] <1HciO :	IJK]^t hN =>?@]^ ;	
VL_u8 2:XC9A_ 4MMjx/0 @7	e	vur9 d<	5

`kon {[		t
W+ ?e|;Y94Qu `i 3Zq6k: l BUCm
DKS)gp a		


B 5k		
y]1|-Y   Ra$%
Np0b1 QGN#k 9$_nR!
ms%A cs:K0 AKy8	c	(sv;
 jf	X
w} WU 
 t	Z

Ba =	h	
ypHoZ J	6

q$/8Val];8 	+e1	
*' Vru	X	B r  "l|G	3vc 		u

T,
 }0\ :K
}YRw  ?(YE"5-v @=J
9}:  ]=f<WZaj 4c
Je>c  		[r 7l;   "a}    My%Ph	
 LwVh u T	$ET* VD3W, m 0C3	n
?[yq&  \x

-6F6  Nwz)		(
U


.CpCLm 6  }+e	
y%    b

R   Hd{/nl				

|  .	q			
y}.#nZ! ; #J6Hj{	C
e
Dz^6cT g w#n/C			\

 ^2N	  L   c%"ZO:G/ WSb
aq2O .T}


~"TSqX?  [R	&
1c*  R!<-KR= *0O		
* 'H'T$
;
GY  Z~71	N	Z	
l j    :H#Co5	
5>$    M9MD			"
rt6^& mSXFw

wMny/2 B%  Og9_%>K`Dev'z		$		In3?|&P] w  jV		
gdL  1F
t; ,
t1 ,_ ?	)i\< 9	UHg Lz6	d	4
s
itwq* B
Ydp{Jf  Q`#"j	~	r,X JVb	'b~ *g		Y
Ax\)&G !kt	 XI|VXv 
!|
 2>VZx  w
Kl5 : cm			
B
[


C{5b"m V! 9.  )/;,60	M	
:

X<" lJCdWu&	C	
H24>L=5\	  0fs >	GXcozhIp[  )_s"i	&a(q 
{WYw4u 9 kVL	9
A

G 13=KW[;  /!erz%Wbn`vt5  K	7
8
=
>
?
@

0m(^_012<IJuv9:rs Q
#0E9)} w kFfQ<:Z VetX	R
* ],2C_		
$1~(! } 
hl

',Fl+Ccx{ / RY;I n\kgv5 W\`G
P
[E#   > lWsy64="C  H !W2=pfY_9
/
x
V!w+#lH m}Y	M	d( H	1n{  s =u&#	S
+4PAHT A3DP	
"-(  `		
S?] i-	%Us ^ 			h
v
2) 	my o !E5]	
j
# ~ ~PS@i		3	\			p

-D| 1Mm	



(Ga,d ) 0  ;9	L
(BMZJS `Ozvb
Z`8Q E:qG<QFx" C  <z%+47Sr}
QA~7gm P T ?o()

^bA  @Zi8FQ6{s ]lhw	o6#.6 4    .,;w  FX]AF

Q\  hH( a 4:~; - N>KK/3F!  X  D^}X7pfg=?&k Z  Ji^
h@A      6Z [DH3 % ? ms(tz	7>z5>X#!b   5JqCy4Z:
*
`
y


t$I \  "O"U,K?m I d>k 	!		WxEfv#  RX3LG7g?`_+	
0
jNCdie   n				Nre) ;
0\sB8< Z		/m k ~>`	l

58j(oh #P 
$
oa W
	| sI	:` q qF	2S  >'9
8! 	I`b  t  L$Og		
#U  [vy|*(	T

,5QBE~$  vzi-				d

]Mx~   bZQ	
!}SpFWN) &&Q	
F)i/      N"8B4Evy			
mq{#.1	 v  U	
^ 5	3
r
T U!a		@[%F+W  H
UJk{ 
:UBc/		
44l- r'VX$ 8 j. F"/Ddy/tq( Uj\dWEBPgk^	

&+Bb   _jx~3OD  G 1<oX	
.
w
UO*@Sz' 	
R\r'! n D4R					
i
o


` ( B *y3F|'AL@f_7   '

:n57z Mg3J. $  ]ro	
G  QF6Bx3^ 	*	
)
_
TDMhd j  	k

/qn p "OV
~  fx{)	'	


7 D#       ua~,u
						
p|E(}    4	q

ZzV0 7 iTcAOfj.	]	


*.c&.Ap! F ;Qw}W		


	TNR& ' A  q22n

9@KIe   Ae&	

Sg#  bv|NV	


)b%-   op@cd
H  .:  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             	                                                                                                                                                                                                                                                        ,                 
                                                                                                                                                   
                        	      	               
   5   #      	   
                        
      	                                                                              !      +         1   	      !         ]   B         [   Z      !         N                                                                                                                                                                                                                                                                                                                                                                                                                                                                              	                                                                                            ,       
                                                     
        	  	     
 5 #  	 
        
  	                          !  +   1 	  !   ] B   [ Z  !   N   d  e  f  f5  i  n  u7+f( p  or233fju
		
:p5 S	- v'	 i
b??	p&
?# J
	
P# K_y$8EE{	
Jy6'c
'	=5 S	-5 S	-5 S	- v'	y
P	  
\

	 0	Gb\
(  p(& ?J
-

n

  	J{
r&<H>u< &uxN
|9g  \

 c_jpUdW
+ &<<x4 ~L]L]' 

@K o =6L#	]g \
 { ]u'O&x	
y  	q)&4x	{   &u{	
x^jNRy	D4	-'f

' ]Ax3o		
@Kx6.s)E{? W |~
eQ& 
g
L	  
FH
B ~=6L#	]]n
    O S W  EP|~)
Abt{	
]aeo?3c]o
{q	uL	
' @K]otO	i	pUD4dW	Gv.c!
osL
u] n=&	
Pnd( j
!){? ]L_ojNC&  q j'-e	v 
;0<
D {a|/V	 uBx3,
			
)
_
}g
H
B 
 !7K>xilz0B   L00Tc		
K' ( A 2'@AKLe{
_uS
+4PA Lc
 K>iB
 !7xlz0p 
\{}Lf 
 #   ;y!$7K6>qx

Pil6z0Bl' ( A  u2Bx3,
			
)
_
'@AKLe}pE	R8^ml$@ yhLw 	D 7E[z/G 1<o
.
w
U* b!F:Lc%P
-ehw G 1<bo
.
w
!U*FI d 	!		Wv  L$Og	
U\<, 2qn 8	/T0	
 %- ;3!P	
m!+ly(icAOfj]	

*Ar|
_	oR<%
Ge3	r%T /%<%G0	
#   ;y$6q

P6l I  Ld$Og 	!			
WvURa1Y X  D^}X7pfg=?&k$  ]ro	
G  ;_y$DN69up8aq	

P6PEPlv1=	 S8h@ap	1
kPQ`ij  W Y CI|~'5h
Y6egy
>?%@v  U	
^|/U~
uAb" D h   10Ts		]


%RLn( B *y3F|'AL@f_7#P 
$
oa	 " D h   S$8h1@ap0Ts~	,				
,
1
]
b


%DRkBP^QL`iLejTn #PJQV`#"b	j	~	 
$
W
'boar	,|~X@=J
9}:   M9MD			"
rt6^  ( B     Qu*ay3F6Bx3F~,^u|
		 	*					
)
_

'ALp|@ETD(fM_7hd} ( B   5v*y3JqzCy4FZ:i-|				
*
`
d
y



'ALt@]Mfx$_7I~d<	5

`konF ;Qw}W		


	TNR&  `   &,@Q*&s	#$>	GXcozhIp[	 6  St8h@akp@}+=JeW			1
z



3k9PQ}%e:`Mi~jy%n\kgv5   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  &<?xml version="1.0"?><Features recycledIDs="9,5" nextValidID="18"><Feature recentID="0" name="lox2272" directionality="1" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1518-1551" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;Cre recombinase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Cre-mediated recombination occurs in the 8-bp core sequence (AAGTATCC) (&lt;u&gt;&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116776/&quot;&gt;Shaw et al., 2021&lt;/u&gt;&lt;/a&gt;). &lt;i&gt;lox2272&lt;/i&gt; sites are compatible with each other, but incompatible with &lt;i&gt;loxP&lt;/i&gt; or &lt;i&gt;loxN&lt;/i&gt; sites (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/9714735&quot;&gt;Lee and Saito, 1988&lt;/a&gt;).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="lox2272" directionality="2" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="660-693" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;Cre recombinase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Cre-mediated recombination occurs in the 8-bp core sequence (AAGTATCC) (&lt;u&gt;&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116776/&quot;&gt;Shaw et al., 2021&lt;/u&gt;&lt;/a&gt;). &lt;i&gt;lox2272&lt;/i&gt; sites are compatible with each other, but incompatible with &lt;i&gt;loxP&lt;/i&gt; or &lt;i&gt;loxN&lt;/i&gt; sites (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/9714735&quot;&gt;Lee and Saito, 1988&lt;/a&gt;).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="loxP" directionality="1" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1602-1635" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;Cre recombinase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Cre-mediated recombination occurs in the 8-bp core sequence (ATGTATGC) (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116776/&quot;&gt;Shaw et al., 2021&lt;/a&gt;).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="loxP" directionality="2" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="744-777" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;Cre recombinase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Cre-mediated recombination occurs in the 8-bp core sequence (ATGTATGC) (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116776/&quot;&gt;Shaw et al., 2021&lt;/a&gt;).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="Factor Xa site" directionality="2" translationMW="473.53" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="2130" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="2056" maxFusedRunOn="2056" detectionMode="exactProteinMatch"><Segment range="2131-2142" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IEGR"/></Q></Feature><Feature recentID="6" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1660-2248" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="hGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2280-2756" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="hSyn promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="175-622" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human synapsin I promoter; confers neuron-specific expression (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/12595892&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/12595892&quot;&gt;Kügler et al., 2003&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="EGFP" directionality="2" translationMW="26941.53" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="786-1499" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Segment name="1a" range="1500-1502" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="1503-1505" color="#05fd14" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;the original enhanced GFP (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot;&gt;Yang et al., 1996&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*"/></Q></Feature><Feature recentID="11" name="Kozak sequence" type="regulatory" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1502-1511" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="12" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3011-3466" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4884-5472" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="AmpR" directionality="1" translationMW="31557.36" type="CDS" allowSegmentOverlaps="0" cleavageArrows="3921" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="3853-3921" color="#ccffcc" type="standard" translated="1"/><Segment range="3922-4713" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="15" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3748-3852" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="AAV2 ITR (alternate)" type="repeat_region" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" prioritize="1" originalSequence="CCTGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCGTCGGGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTAGGGGTTCCT"><Segment range="1-130" color="#ffcc99" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Functional equivalent of wild-type AAV2 ITR&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="AAV2 ITR" type="repeat_region" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" prioritize="1" originalSequence="CTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTAGGGGTTCCT"><Segment range="2796-2925" color="#ffcc99" type="standard"/></Feature></Features>
  	<?xml version="1.0"?><Primers nextValidID="12"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="Amp-R" sequence="ATAATACCGCGCCACATAGC" description="Ampicillin resistance gene, reverse primer"><BindingSite location="4070-4089" boundStrand="1" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="4070-4089" bases="ATAATACCGCGCCACATAGC"/></BindingSite><BindingSite simplified="1" location="4070-4089" boundStrand="1" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="4070-4089" bases="ATAATACCGCGCCACATAGC"/></BindingSite></Primer><Primer recentID="1" name="EGFP-C" sequence="CATGGTCCTGCTGGAGTTCGTG" description="EGFP, forward primer"><BindingSite location="830-851" boundStrand="1" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="830-851" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite><BindingSite simplified="1" location="830-851" boundStrand="1" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="830-851" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite></Primer><Primer recentID="2" name="EGFP-N" sequence="CGTCGCCGTCCAGCTCGACCAG" description="EGFP, reverse primer"><BindingSite location="1438-1459" boundStrand="0" annealedBases="CGTCGCCGTCCAGCTCGACCAG" meltingTemperature="67"><Component hybridizedRange="1438-1459" bases="CGTCGCCGTCCAGCTCGACCAG"/></BindingSite><BindingSite simplified="1" location="1438-1459" boundStrand="0" annealedBases="CGTCGCCGTCCAGCTCGACCAG" meltingTemperature="67"><Component hybridizedRange="1438-1459" bases="CGTCGCCGTCCAGCTCGACCAG"/></BindingSite></Primer><Primer recentID="3" name="EXFP-R" sequence="GTCTTGTAGTTGCCGTCGTC" description="For distinguishing EGFP variants, reverse primer"><BindingSite location="1179-1198" boundStrand="0" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="1179-1198" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite><BindingSite simplified="1" location="1179-1198" boundStrand="0" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="1179-1198" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite></Primer><Primer recentID="4" name="F1ori-F" sequence="GTGGACTCTTGTTCCAAACTGG" description="F1 origin, forward primer"><BindingSite location="3307-3328" boundStrand="0" annealedBases="GTGGACTCTTGTTCCAAACTGG" meltingTemperature="57"><Component hybridizedRange="3307-3328" bases="GTGGACTCTTGTTCCAAACTGG"/></BindingSite><BindingSite location="5224-5233" boundStrand="1" annealedBases="TCCAACTGG" meltingTemperature="13"><Component bases="GTGGACTCTTGT"/><Component hybridizedRange="5229-5233" bases="TCCAA"/><Component bases="A"/><Component hybridizedRange="5224-5227" bases="CTGG"/></BindingSite><BindingSite simplified="1" location="3307-3328" boundStrand="0" annealedBases="GTGGACTCTTGTTCCAAACTGG" meltingTemperature="57"><Component hybridizedRange="3307-3328" bases="GTGGACTCTTGTTCCAAACTGG"/></BindingSite><BindingSite simplified="1" location="5224-5233" boundStrand="1" annealedBases="TCCAACTGG" meltingTemperature="13"><Component bases="GTGGACTCTTGT"/><Component hybridizedRange="5229-5233" bases="TCCAA"/><Component bases="A"/><Component hybridizedRange="5224-5227" bases="CTGG"/></BindingSite></Primer><Primer recentID="5" name="F1ori-R" sequence="AGGGAAGAAAGCGAAAGGAG" description="F1 origin, reverse primer"><BindingSite location="3097-3116" boundStrand="1" annealedBases="AGGGAAGAAAGCGAAAGGAG" meltingTemperature="57"><Component hybridizedRange="3097-3116" bases="AGGGAAGAAAGCGAAAGGAG"/></BindingSite><BindingSite simplified="1" location="3097-3116" boundStrand="1" annealedBases="AGGGAAGAAAGCGAAAGGAG" meltingTemperature="57"><Component hybridizedRange="3097-3116" bases="AGGGAAGAAAGCGAAAGGAG"/></BindingSite></Primer><Primer recentID="6" name="hGH-PA-R" sequence="CCAGCTTGGTTCCCAATAGA" description="Human growth hormone terminator, reverse primer"><BindingSite location="2478-2497" boundStrand="1" annealedBases="CCAGCTTGGTTCCCAATAGA" meltingTemperature="55"><Component hybridizedRange="2478-2497" bases="CCAGCTTGGTTCCCAATAGA"/></BindingSite><BindingSite simplified="1" location="2478-2497" boundStrand="1" annealedBases="CCAGCTTGGTTCCCAATAGA" meltingTemperature="55"><Component hybridizedRange="2478-2497" bases="CCAGCTTGGTTCCCAATAGA"/></BindingSite></Primer><Primer recentID="7" name="pBR322ori-F" sequence="GGGAAACGCCTGGTATCTTT" description="pBR322 origin, forward primer"><BindingSite location="5372-5391" boundStrand="0" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="5372-5391" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite><BindingSite simplified="1" location="5372-5391" boundStrand="0" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="5372-5391" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite></Primer><Primer recentID="8" name="pBRforEco" sequence="AATAGGCGTATCACGAGGC" description="pBR322 vectors, upsteam of EcoRI site, forward primer"><BindingSite location="3661-3679" boundStrand="1" annealedBases="AATAGGCGTATCACGAGGC" meltingTemperature="57"><Component hybridizedRange="3661-3679" bases="AATAGGCGTATCACGAGGC"/></BindingSite><BindingSite simplified="1" location="3661-3679" boundStrand="1" annealedBases="AATAGGCGTATCACGAGGC" meltingTemperature="57"><Component hybridizedRange="3661-3679" bases="AATAGGCGTATCACGAGGC"/></BindingSite></Primer><Primer recentID="9" name="pGEX 3'" sequence="CCGGGAGCTGCATGTGTCAGAGG" description="pGEX vectors, reverse primer"><BindingSite location="3601-3623" boundStrand="0" annealedBases="CCGGGAGCTGCATGTGTCAGAGG" meltingTemperature="66"><Component hybridizedRange="3601-3623" bases="CCGGGAGCTGCATGTGTCAGAGG"/></BindingSite><BindingSite simplified="1" location="3601-3623" boundStrand="0" annealedBases="CCGGGAGCTGCATGTGTCAGAGG" meltingTemperature="66"><Component hybridizedRange="3601-3623" bases="CCGGGAGCTGCATGTGTCAGAGG"/></BindingSite></Primer><Primer recentID="10" name="pRS-marker" sequence="CGGCATCAGAGCAGATTGTA" description="pRS vectors, use to sequence yeast selectable marker"><BindingSite location="3482-3501" boundStrand="1" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="3482-3501" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite><BindingSite simplified="1" location="3482-3501" boundStrand="1" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="3482-3501" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite></Primer><Primer recentID="11" name="WPRE-R" sequence="CATAGCGTAAAAGGAGCAACA" description="WPRE, reverse primer"><BindingSite location="1712-1732" boundStrand="1" annealedBases="CATAGCGTAAAAGGAGCAACA" meltingTemperature="55"><Component hybridizedRange="1712-1732" bases="CATAGCGTAAAAGGAGCAACA"/></BindingSite><BindingSite simplified="1" location="1712-1732" boundStrand="1" annealedBases="CATAGCGTAAAAGGAGCAACA" meltingTemperature="55"><Component hybridizedRange="1712-1732" bases="CATAGCGTAAAAGGAGCAACA"/></BindingSite></Primer></Primers>
  <Notes>
<UUID>7603d1e6-5491-4a3f-9a9b-d96bd7d0b759</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>AAV_DIO_EGFP_WPRE</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>Double floxed EGFP under the control of human synapsin promoter.</Description>
<Created UTC="4:0:0">2024.8.25</Created>
<LastModified UTC="15:46:18">2024.10.22</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<References>
<Reference type="Journal Article" journalName="Unpublished" journal="Unpublished" title="Roth lab DREADDs" authors="Bryan Roth"/>
</References>
</Notes>
  Y              Unique 6+ Cutters                                                                                            d     THO  d              E              Y? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
