	   SnapGene      gggcgatggcccactacgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttccttgtagttaatgattaacccgccatgctacttatctacgtagccatgctctaggaagatcgtaggttaattaatctagactgcagagggccctgcgtatgagtgcaagtgggttttaggaccaggatgaggcggggtgggggtgcctacctgacgaccgaccccgacccactggacaagcacccaacccccattccccaaattgcgcatcccctatcagagagggggaggggaaacaggatgcggcgaggcgcgtgcgcactgccagcttcagcaccgcggacagtgccttcgcccccgcctggcggcgcgcgccaccgccgcctcagcactgaaggcgcgctgacgtcactcgccggtcccccgcaaactccccttcccggccaccttggtcgcgtccgcgccgccgccggcccagccggaccgcaccacgcgaggcgcgagataggggggcacgggcgcgaccatctgcgctgcggcgccggcgactcagcgctgcctcagtctgcggtgggcagcggaggagtcgtgtcgtgcctgagagcgcagtcgagaggatccataacttcgtataatgtatgctatacgaagttatgggtcgatggtgagatctggactagagggtcgatggtgatgcttggatcaaaataggaagaatcccaatgataacttcgtataaagtatcctatacgaagttattcacttcgctgtcatcatttgtacaaactcttcgtagtttacctgaccatccccatcgatgtctgctaccctgatcatttcatcaacctcctcatctgttaacttctctccaaggtctgtcatcacgtggcgaagctctgctgcgccgatgtagccattgccgtccttatcaaacacgcggaacgcttctctaatttcctcttcactgtctgtgtcattcatttttcttgccatcatcgtcaggaactcagggaagtcgaatgtgccgtcaccgtcggcatctacttcattgatcatgtcctgcagctctgcttctgtggggttctgccccagagaccgcatcaccgtccccagctccttggttgttatcgtcccatccccgtccttgtcaaatagggagaaagcctctttaaattctgcgatctgctcttcagtcagttggtccagaataaacgccggcatccagcccattgtcttcttctgcattacggggccgtcgggggggaagttggtgccgcgcagcttcaccttgtagatgaatacgccgtcctccagggaggtgtcctgaaacacaaccacggcgccgccgtcctcgaagttcatcacgcgctcccacttgaagccctcggggaaggactgcttatagtagtcggggatgtcggcggggtgcttagtgaaggccctggagccgtacatgaactgaggggacaggatgtcccaggagaagggcagggggccacccttggtcaccgtcagcttggcggtctgggtaccctcgtaggggcggccctcgccctcgccctcgatctcgaactcgtggccgtttacggagccctccatgtgcaccttgtagcgcatgaactccttgatcactgcctcgcccttgctcaccatactaccaccagttcctcccttgtacagctcgtccatgccgccggtggagtggcgcgccacggagcgttcgtactgttccaccacggtgtagtcctcgttgtgggaggtgatgtccaacttgcggtcgacgttgtaggcgccgggcatctgcacgggcttcttggcaatataggtggtcttgaagtccgccaggtagcggccgccgtccttcaggcgcagggccatcttaatgtcgcccttcagcacgccgtcctcggggtacaaccgctccacagaccaagatcttgagctcagccgacctatagctctgactgcgtgacctgccttattccacttacgacgtgatgagtcgaccattgatgagtcgaccattgttgcgagatccttatcgtcatcgtcgtacagatcccgacccatttgctgtccaccagtcatgctagccataccatgatgatgatgatgatgagaacctcctccagtcttgttaatatcaagtccagccaacttaagagcaagctcgttctgcagcatataacttcgtatagcatacattatacgaagttatgggtcgatggtgatgcttggcaattcgggtcgatggtgaagcattggtcttcctattttgatataacttcgtataggatactttatacgaagttatgaattcgatatcaagcttatcgataatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcatcgataccgtcgacccgggcggccgctcataaagctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtccggactgtacaattgcattcattttatgtttcaggttcagggggagatgtgggaggttttttaaagcaagtaaaacctctacaaatgtggtaaaatcgataaggatcttcctagagcatggctacgtagataagtagcatggcgggttaatcattaactacaaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatca  1  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,GDGCHC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,GCCGGC,CAATTG,ACGCGT,TCCRAC,GTYGGA,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,RGCR,YGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                   $                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         	                                                                                                                                                                                                                         	                                              *                                                                                                      
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   4      
         	                                                         	                                                    *   #   	      4         !         ^   {      B         R   Q      %         M                                                                                                                                                                        $                                                                                                                                                                                                                                                                                             	                                                                               	                    *                                    
                             	  	     
 4  
   	                   	                  * # 	  4   !   ^ {  B   R Q  %   M   5  6  6  7  :  ?  H%*  g83gG~Ti]8&It<<RF}_{rz0	1
E Wt	Rt	R4^1y      :WW
(	1		oI>/t<<t<_<t<<F	9?#w}*55 		Ze	#Ibb#NX5fsT	c	
6	z5 Lxz[ 		 ";#


&jfY	J		vv[f 	vW 	
OqR[
gx

n #	
2  F	5 J V L$cx&Vz[	kgW 	v$		4.I k[fA"F^0H= Sd%dw-u !W@A+P	+\YqmEA^=,@$JVu   &:AEIW@+		E)	}(0Hk	s		WEP [f ,	

$&	\-
sT	c	
 sT	c		vp	!i4		
3Ju W@h:<
<sT	c			.4IcZFGl 	g	>WEt		QI`j8#G<VTXeF >

Bew[^,t (5$Zc2d WZ~k[\fg9 ?V4a5
O
 d5$c2
[^, >
Bewt `.e  12
L
8 f)   >WQT{

Bewz[^,tA&[\fg89#G<V TXe?VD878

i
Wd		o`;@E-p	pR52:d %DvFf?JzgRp5	p2rpPhEyf\Vc*l kIZ ) &Bb~-MR	a	Z`7<nlhuI		)CJH *	4i15kyI MX	

lbV  &4  c3	)hB| JN-A%SV\
ZKWR	a	7n`< c3	BMZ  WQT{zA&PhEyf\Vcrp0
S#+Z2@   BHNnu*},a*,7n   X*MRUf| 		Om'  &B+rpb~-7zA,{=	 f =l	'		
ka      ACOt~+-`+Ghv8o 
'	#_?	t		XFQYq )V<Ze XZ~$k v \g":XaA &@eW/		ez X f t )=Vd<Z_Je (Xl		'				
ZA~,h$v	k`_kWa  ? x         _jqAqsCQq*6	

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T
fn1cx}6F|<[Z]sx +.7wCYx:n*7         19DHLY~N  PSV9	1^"?z.	9			:




 BDHQp
(:G]HPXdi,wSn7s*0FNTp%  am/$				




 
*
j
t




5M	ezN1T>uR6 } wK	jgtl#&[
w'vv ;\g8T":X]a5AH#G 7				<JV*TXe}&@9oeW `v   \g9j":Xa"jrA$>HSw 

=dWUHYf&@Xeli5eW	w:B


+?T		
-><Up3 T?q*7_P|%47{\ 		

-x 4 f } =uS@l`w	!	'	6	K			

jUgtZ&;l8k|#a1 /ztU	d	0
J


P(   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  .<?xml version="1.0"?><Features nextValidID="19"><Feature recentID="0" name="lox2272" directionality="2" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2668-2701" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;Cre recombinase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Cre-mediated recombination occurs in the 8-bp core sequence (AAGTATCC) (&lt;u&gt;&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116776/&quot;&gt;Shaw et al., 2021&lt;/u&gt;&lt;/a&gt;). &lt;i&gt;lox2272&lt;/i&gt; sites are compatible with each other, but incompatible with &lt;i&gt;loxP&lt;/i&gt; or &lt;i&gt;loxN&lt;/i&gt; sites (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/9714735&quot;&gt;Lee and Saito, 1988&lt;/a&gt;).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="lox2272" directionality="1" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1113-1146" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;Cre recombinase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Cre-mediated recombination occurs in the 8-bp core sequence (AAGTATCC) (&lt;u&gt;&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116776/&quot;&gt;Shaw et al., 2021&lt;/u&gt;&lt;/a&gt;). &lt;i&gt;lox2272&lt;/i&gt; sites are compatible with each other, but incompatible with &lt;i&gt;loxP&lt;/i&gt; or &lt;i&gt;loxN&lt;/i&gt; sites (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/9714735&quot;&gt;Lee and Saito, 1988&lt;/a&gt;).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="loxP" directionality="2" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2572-2605" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;Cre recombinase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Cre-mediated recombination occurs in the 8-bp core sequence (ATGTATGC) (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116776/&quot;&gt;Shaw et al., 2021&lt;/a&gt;).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="loxP" directionality="1" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1009-1042" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;Cre recombinase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Cre-mediated recombination occurs in the 8-bp core sequence (ATGTATGC) (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116776/&quot;&gt;Shaw et al., 2021&lt;/a&gt;).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="Factor Xa site" directionality="2" translationMW="473.53" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="3196" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="3122" maxFusedRunOn="3122" detectionMode="exactProteinMatch"><Segment range="3197-3208" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IEGR"/></Q></Feature><Feature recentID="5" name="Xpress™ tag" directionality="2" translationMW="997.97" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="2424" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="1147" maxFusedRunOn="1147" detectionMode="exactProteinMatch"><Segment name="enterokinase site" range="2425-2439" color="#cc99b2" type="standard" translated="1"/><Segment range="2440-2448" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Xpress™ epitope tag, including an enterokinase recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="DLY,DDDDK"/></Q></Feature><Feature recentID="6" name="6xHis" directionality="2" translationMW="840.86" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="1147" maxFusedRunOn="1147" detectionMode="exactProteinMatch"><Segment range="2488-2505" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;6xHis affinity tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="HHHHHH"/></Q></Feature><Feature recentID="7" name="T7 tag (gene 10 leader)" directionality="2" translationMW="1098.29" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="1147" maxFusedRunOn="1147"><Segment range="2452-2484" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promotes efficient translation in &lt;i&gt;E. coli&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;leader peptide from bacteriophage T7 gene &lt;i&gt;10&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MASMTGGQQMG"/></Q></Feature><Feature recentID="8" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2726-3314" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3355-3562" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="hSyn promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="549-996" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human synapsin I promoter; confers neuron-specific expression (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/12595892&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/12595892&quot;&gt;Kügler et al., 2003&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="calmodulin-binding peptide" directionality="2" translationMW="2163.52" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="1147" maxFusedRunOn="1147" detectionMode="exactProteinMatch"><Segment range="2326-2382" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;calmodulin-binding peptide from avian smooth muscle myosin light chain kinase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="RRKWNKAGHAVRAIGRLSS"/></Q></Feature><Feature recentID="12" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5848-248" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4098-4686" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="calmodulin" directionality="2" translationMW="16597.28" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="3" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="1147-1590" color="#993366" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;calcium-binding protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGDGTFDFPEFLTMMARKMNDTDSEEEIREAFRVFDKDGNGYIGAAELRHVMTDLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK*"/></Q></Feature><Feature recentID="15" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5648" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4857-5648" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5649-5717" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="16" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5718-5822" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="AAV2 ITR" type="repeat_region" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" prioritize="1" originalSequence="CTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTAGGGGTTCCT"><Segment range="3732-3861" color="#ffcc99" type="standard"/></Feature><Feature recentID="18" name="AAV2 ITR" type="repeat_region" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" prioritize="1" originalSequence="CTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTAGGGGTTCCT"><Segment range="320-449" color="#ffcc99" type="standard"/></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="6"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="Amp-R" sequence="ATAATACCGCGCCACATAGC" description="Ampicillin resistance gene, reverse primer"><BindingSite location="5479-5498" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="5479-5498" bases="ATAATACCGCGCCACATAGC"/></BindingSite><BindingSite simplified="1" location="5479-5498" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="5479-5498" bases="ATAATACCGCGCCACATAGC"/></BindingSite></Primer><Primer recentID="1" name="F1ori-F" sequence="GTGGACTCTTGTTCCAAACTGG" description="F1 origin, forward primer"><BindingSite location="4335-4344" boundStrand="0" annealedBases="TCCAACTGG" meltingTemperature="13"><Component bases="GTGGACTCTTGT"/><Component hybridizedRange="4335-4339" bases="TCCAA"/><Component bases="A"/><Component hybridizedRange="4341-4344" bases="CTGG"/></BindingSite><BindingSite location="5984-6005" boundStrand="1" annealedBases="GTGGACTCTTGTTCCAAACTGG" meltingTemperature="57"><Component hybridizedRange="5984-6005" bases="GTGGACTCTTGTTCCAAACTGG"/></BindingSite><BindingSite simplified="1" location="4335-4344" boundStrand="0" annealedBases="TCCAACTGG" meltingTemperature="13"><Component bases="GTGGACTCTTGT"/><Component hybridizedRange="4335-4339" bases="TCCAA"/><Component bases="A"/><Component hybridizedRange="4341-4344" bases="CTGG"/></BindingSite><BindingSite simplified="1" location="5984-6005" boundStrand="1" annealedBases="GTGGACTCTTGTTCCAAACTGG" meltingTemperature="57"><Component hybridizedRange="5984-6005" bases="GTGGACTCTTGTTCCAAACTGG"/></BindingSite></Primer><Primer recentID="2" name="F1ori-R" sequence="AGGGAAGAAAGCGAAAGGAG" description="F1 origin, reverse primer"><BindingSite location="141-160" boundStrand="0" annealedBases="AGGGAAGAAAGCGAAAGGAG" meltingTemperature="57"><Component hybridizedRange="141-160" bases="AGGGAAGAAAGCGAAAGGAG"/></BindingSite><BindingSite simplified="1" location="141-160" boundStrand="0" annealedBases="AGGGAAGAAAGCGAAAGGAG" meltingTemperature="57"><Component hybridizedRange="141-160" bases="AGGGAAGAAAGCGAAAGGAG"/></BindingSite></Primer><Primer recentID="3" name="mCherry-F" sequence="CCCCGTAATGCAGAAGAAGA" description="mCherry, forward primer"><BindingSite location="1453-1462" boundStrand="1" annealedBases="CAGAAGAGA" meltingTemperature="13"><Component bases="CCCCGTAATG"/><Component hybridizedRange="1457-1462" bases="CAGAAG"/><Component bases="A"/><Component hybridizedRange="1453-1455" bases="AGA"/></BindingSite><BindingSite location="1619-1638" boundStrand="1" annealedBases="CCCCGTAATGCAGAAGAAGA" meltingTemperature="54"><Component hybridizedRange="1619-1638" bases="CCCCGTAATGCAGAAGAAGA"/></BindingSite><BindingSite simplified="1" location="1453-1462" boundStrand="1" annealedBases="CAGAAGAGA" meltingTemperature="13"><Component bases="CCCCGTAATG"/><Component hybridizedRange="1457-1462" bases="CAGAAG"/><Component bases="A"/><Component hybridizedRange="1453-1455" bases="AGA"/></BindingSite><BindingSite simplified="1" location="1619-1638" boundStrand="1" annealedBases="CCCCGTAATGCAGAAGAAGA" meltingTemperature="54"><Component hybridizedRange="1619-1638" bases="CCCCGTAATGCAGAAGAAGA"/></BindingSite></Primer><Primer recentID="4" name="pBR322ori-F" sequence="GGGAAACGCCTGGTATCTTT" description="pBR322 origin, forward primer"><BindingSite location="4177-4196" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="4177-4196" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite><BindingSite simplified="1" location="4177-4196" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="4177-4196" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite></Primer><Primer recentID="5" name="WPRE-R" sequence="CATAGCGTAAAAGGAGCAACA" description="WPRE, reverse primer"><BindingSite location="2778-2798" boundStrand="1" annealedBases="CATAGCGTAAAAGGAGCAACA" meltingTemperature="55"><Component hybridizedRange="2778-2798" bases="CATAGCGTAAAAGGAGCAACA"/></BindingSite><BindingSite simplified="1" location="2778-2798" boundStrand="1" annealedBases="CATAGCGTAAAAGGAGCAACA" meltingTemperature="55"><Component hybridizedRange="2778-2798" bases="CATAGCGTAAAAGGAGCAACA"/></BindingSite></Primer></Primers>
  <Notes>
<UUID>7a891a1f-c27e-44b2-a232-102d3e38f379</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pAAV-Syn-DIO-PinkyCaMP</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under Syn promoter in a Cre-dependent manner (DIO, double floxed inverted open reading frame).</Description>
<Created UTC="5:0:0">2025.12.15</Created>
<LastModified UTC="5:0:0">2025.12.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<References>
<Reference authors="Fink R, Imai S, Gockel N, Lauer G, Renken K, Wietek J, Lamothe-Molina PJ, Furhmann F, Mittag M, Ziebarth T, Canziani A, Kubitschke M, Kistmacher V, Kretschmer A, Sebastian E, Schmitz D, Terai T, Grundemann J, Hassan S, Patriarchi T, Reiner A, Fuhrmann M, Campbell RE, Masseck OA" journal="bioRxiv [Preprint]. 2025 Jan 11:2024.12.16.628673. doi: 10.1101/2024.12.16.628673." journalName="bioRxiv [Preprint]. 2025 Jan 11:2024.12.16.628673. doi: 10.1101/2024.12.16.628673." pubMedID="39763884" title="PinkyCaMP a mScarlet-based calcium sensor with exceptional brightness, photostability, and multiplexing capabilities." type="Journal Article"/>
</References>
</Notes>
  Y              Unique 6+ Cutters                                                                                            d     THO  d              E              Y? P                                                                                                                                                           #   <7zXZ  ִF !   t/ []   S.( .~}    YZ   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
