	   SnapGene      CCTGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCGTCGGGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTAGGGGTTCCTGCGGCCGCAACGCGTTTAGAACAATGGCTGGCCCATAGTAAATGCCGTGTTAGTGTGTTAGTTGCTGTTCTTCCACGTCAGAAGAGGCACAGACAAATTACCACCAGGTGGCGCTCAGAGTCTGCGGAGGCATCACAACAGCCCTGAATTTGAATCCTGCTCTGCCACTGCCTAGTTGAGACCTTTTACTACCTGACTAGCTGTTTGTGTATTTTAGGTGTTTGTTTATCTATACCTCTGAGAAAGCCATTTTTTTTAACATGAAGAAGTATAAAATATTGTGTGTGAAGATTAAGCCCTTTCTTGCTCTATGTATTTGAGCCTAGAAAAAGACACACTCCCAAGAAATTAGAATAAAGGGAAAAATGACTTTGAGATGTACTTTCTTAAAATAACTGAAGTAATGACCGCATGAGCCAGCTGATGGGTTTTTAAATGGATACGTTTCTATCAGCCTGCTGCTTGATGCCAGAGCCAAATATATATGGAGTGTATTATATCATATCCTGTAGGCAGGAGACTGTTGGGGCATTGGGGGACTTAGAGAGGTGAAGTGGCAGGCTTGGCACAGGAATTAACAGCATGGCTACCGTCAACCAGAATTCGATATCATAATCAACCATAGGTACCGAGCTCGGGATTCAGCCGGGAGCTTAGGGAGGGGAGGTCACTTCATAAGGGCCTGGGGGGGGAGTTGGAGCCACGAGTCGTCCAGCCGGAGCCCCGTGTGGCTGAGCTCCGGCCTCAGAAGCATCCCCGGGTTGGATCCTTCGAAGCTAGCGCTACCGGTCATAACTTCGTATAGGATACTTTATACGAAGTTATCCTCGAGTACCGGTCACGCGTACGCAAGCTTGATAACTTCGTATAGCATACATTATACGAAGTTATCCTAGGTTAGGAGCCACCGGAGCCGCCGGTGGAGTGGCGGGCCACGGAGCGTTCGTACTGTTCCACCACGGTGTAGTCCTCGTTGTGGGATGTGATGTCCAACTTGCGGTCGATGTTGAAGGCGCCGGGCATCTGCACGGGCTTCTTGGCCCTGTAGGTGGTCTTGAAGTCCGCCAGGTAGCGGCCGCCGTCCTTCAGGCGCAGGGCCATCTTAATGTCGCCCTTCAGCACGACGTCCTCGGGGTACAACCGCTCGGTGGATGCTTCCCAGCCCATTGTCTTCTTCTGCATTACGGGGCCGTCAGGAGGGAAGTTGGTGCCGCGGAGCTTCACCTTGTAGATCAGGGTGCCGTCCTCCAGGGAGGTGTCCTGGGCCACGGACACGGCGCCGCCGTCCTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACTGCTTCCAGTAGTCGGGGATGTCGGCGGGGTGCTTCGTGAAGGCCCTGGAGCCGTACATGAACTGAGGGGACAGGATGTCCCAGGAGAAGGGCAGGGGGCCACCCTTGGTCACCCTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGGCCGTTCATGGAGCCCTCCATGTGCACCTTGAACCGCATGAACTCCTTGATCACTGCCTCGGTGCTATCCATCACGTTGATGTCGATCTGGTCCAGGGGGATGTACTCGCCCTCGCTGGTGATCCTGCTCTTGGCGGCCGCCACTTTATCTTCCTCTGTCACGAAGGCCAGCACGTCCACCTTGCGTCCCAGAAACTGGGAGCTCACTGGTCTCCTGATATCGCCGTACATAATGATATGCTCGTGAATTTTGATCCGCAGGAAGTGCCCCAGCAGTCCCCAAATATTCTTGGACAGCAGGTCGGCGATGGTATGGCCAATAGTGGACCCAGCCACGCTCAGGGTGTGCATGCCTTCAGGCCCAAAGATGAACAGGCCGGGAAACATCAGCCAACTAGAATAGTACACGGCAGTCATGGCCAGCACCAGTTTCTTACAGCCGCCGGCAGGCATGATATAGTAAGACTCCACGTAGATGATTCCAGCGTTGTAGAATGTCTGGGTTCCATACACCAGGCCGATACAGTAAAACAGCCACTTCACCCACCCAGTGGCCAGAGCGGCTGTCACTCCCATGCAAATAGTTCCCAGGTCGGACACCAGCAGAGCCATTGTCCGCTTATTGTATGCCTCACTCAGGCCGGTGATGTTAGACAGATGAATCAGGATCACGGGACATGTCAGCAGCCATTCGGCATATCTCAGCCATGGGGTAATGTTCCCTCCGTACAGGAACAGCATAGCAGGACTGGTGAACTCGAAATAGATCTCCAGAATCACTTTGATCAGCTCCACGGAGCACACGTACACTTCCTCCCATCCCACGCTGGCCTTATAGGCGTGCCAGCCGTAGAAAGCCAGAAACAGGGCAGACAGGAACACGGCAAACCACTGGAAGCCCCTAGCTGTTTCCTGCTCCCACAGGGCCCCATAGTAGATGTAGATGGAGCTGCACTGTTCCTCATACCCATGCACGGGGTGGTAGGGTGGATTCACTTGATATCCAGCTGGGGCATGGTACTCACTGCCTCCGGCAGAATCGTTTCCAGGTGGGGCGCTGTGAGCGTCTGGAGCAGGAGCTCCGCCCATGGTGGCTCTAGAATAACTTCGTATAAAGTATCCTATACGAAGTTATAGGTACCTGAGCTCAACATCGATAGCACTAGTATAACTTCGTATAATGTATGCTATACGAAGTTATGTCGACATCATAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTAGTTCTTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGCTCGAGAGATCTTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGAGATCTCACGTGCGGACCGAGCGGCCGCAGGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAGTGAGCGAGCGAGCGCGCAGCTGCCTGCAGGGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATACGTCAAAGCAACCATAGTACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCTTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTTGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACTCTATCTCGGGCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGTCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGGGAATTTTAACAAAATATTAACGTTTACAATTTTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGT  1  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,GDGCHC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,GCCGGC,CAATTG,ACGCGT,TCCRAC,GTYGGA,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,RGCR,YGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                        %                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        	                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
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target=&amp;quot;_blank&amp;quot;&amp;gt;J Biol Chem. 267:16330 (1992)&amp;lt;/a&amp;gt;; &amp;lt;a href=&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;Nat Protoc. 7:171 (2012)&amp;lt;/a&amp;gt;&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/color=#da3bb5&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="AAV2 ITR" type="repeat_region" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3347-3476" color="#a6acb3" type="standard"/><Q name="label"><V text="AAV2 ITR"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;/Application Notes=Allows rescue of virus from recombinant plasmid and replication of the viral genome; this ITR is identical to that of the wild-type AAV2 genome.&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/References=J Virol. 34:402 (1980)&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/color=#e4b930&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="f1 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3562-4017" color="#ffff00" type="standard"/><Q name="label"><V text="f1 ori"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;/color=#7a00b5&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="AmpR" directionality="1" translationMW="31557.36" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4404-5264" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="label"><V text="AmpR"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;/Application Notes=Allows E. coli to be resistant to ampicillin.&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/Full Name=Ampicillin resistance gene&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/References=Proc Natl Acad Sci U S A. 75: 3737 (1978)&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/Selection Drug=Ampicillin &lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/Subtype=E. coli selection marker&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/Usage=Positive selection&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/color=#05696d&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="16" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5435-6023" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="label"><V text="ori"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;/Application Notes=Facilitates plasmid replication in E. coli; regulates high-copy plasmid number (500-700).&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/Full Name=pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/References=Mol Microbiol. 6:3385 (1992)&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/Subtype=E. coli replication_origin&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/color=#f54c6b&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/></Primers>
  `<Notes>
<UUID>370591a1-ea04-4df0-887e-e1a045f2e777</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="22:0:0">2026.7.20</Created>
<LastModified UTC="22:0:0">2026.7.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<Comments>Vector Name: pAAV-hsA2-eHGT140h-minRho_Lox2272/LoxP/rev(CheRiff:mScarlet3)/Lox2272/LoxP_WPRE3-bGH(pA)&lt;br>Vector Type: Mammalian Gene Expression AAV Vector&lt;br>Plasmid Copy Number:&amp;nbsp;&amp;nbsp;High&lt;br>Antibiotic Resistance:&amp;nbsp;&amp;nbsp;Ampicillin</Comments>
</Notes>
  Y      K         Unique 6+ Cutters                                                                                            d     THO               E              Y? P                                                                                                                                                           #   <7zXZ  ִF !   t/ []   S.( .~}    YZ   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
