	   SnapGene      CCTGCAGGCAGCTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCGTCGGGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTAGGGGTTCCTGCGGCCGCAACGCGTTTAGAACAATGGCTGGCCCATAGTAAATGCCGTGTTAGTGTGTTAGTTGCTGTTCTTCCACGTCAGAAGAGGCACAGACAAATTACCACCAGGTGGCGCTCAGAGTCTGCGGAGGCATCACAACAGCCCTGAATTTGAATCCTGCTCTGCCACTGCCTAGTTGAGACCTTTTACTACCTGACTAGCTGTTTGTGTATTTTAGGTGTTTGTTTATCTATACCTCTGAGAAAGCCATTTTTTTTAACATGAAGAAGTATAAAATATTGTGTGTGAAGATTAAGCCCTTTCTTGCTCTATGTATTTGAGCCTAGAAAAAGACACACTCCCAAGAAATTAGAATAAAGGGAAAAATGACTTTGAGATGTACTTTCTTAAAATAACTGAAGTAATGACCGCATGAGCCAGCTGATGGGTTTTTAAATGGATACGTTTCTATCAGCCTGCTGCTTGATGCCAGAGCCAAATATATATGGAGTGTATTATATCATATCCTGTAGGCAGGAGACTGTTGGGGCATTGGGGGACTTAGAGAGGTGAAGTGGCAGGCTTGGCACAGGAATTAACAGCATGGCTACCGTCAACCAGAATTCGATATCATAATCAACCATAGGTACCGAGCTCGGGATTCAGCCGGGAGCTTAGGGAGGGGAGGTCACTTCATAAGGGCCTGGGGGGGGAGTTGGAGCCACGAGTCGTCCAGCCGGAGCCCCGTGTGGCTGAGCTCCGGCCTCAGAAGCATCCCCGGGTTGGATCCTTCGAAGCTAGCGCTACCGGTCATAACTTCGTATAGGATACTTTATACGAAGTTATCCTCGAGTACCGGTCACGCGTACGCAAGCTTGATAACTTCGTATAGCATACATTATACGAAGTTATCCTAGGTTACTTGTACAGCTCGTCCATGCCGAGAGTGATCCCGGCGGCGGTCACGAACTCCAGCAGGACCATGTGATCGCGCTTCTCGTTGGGGTCTTTGCTCAGCTTGGACTGGTAGCTCAGGTAGTGGTTGTCGGGCAGCAGCACGGGGCCGTCGCCGATGGGGGTGTTCTGCTGGTAGTGGTCGGCGAGCTGCACGCCGCCGTCCTCGATGTTGTGGCGGATCTTGAAGTTGGCCTTGATGCCGTTCTTCTGCTTGTCGGCGGTGATATAGACGTTGTGGCTGTTGTAGTTGTACTCCAGCTTGTGCCCCAGGATGTTGCCGTCCTCCTTGAAGTCGATGCCCTTCAGCTCGATGCGGTTCACCAGGGTGTCGCCCTCGAACTTCACCTCGGCGCGGGTCTTGTAGTTGCCGTCGTCCTTGAAGAAGATGGTGCGCTCCTGGACGTAGCCTTCGGGCATGGCGGACTTGAAGAAGTCGTGCTGCTTCATGTGGTCGGGGTAGCGGGCGAAGCACTGCACGCCGTAGCCCAGGGTGGTCACGAGGGTGGGCCAGGGCACGGGCAGCTTGCCGGTGGTGCAGATCAGCTTCAGGGTCAGCTTGCCGTAGGTGGCATCGCCCTCGCCCTCGCCGGACACGCTGAACTTGTGGCCGTTTACGTCGCCGTCCAGCTCGACCAGGATGGGCACCACCCCGGTGAACAGCTCCTCGCCCTTGCTCACCATGGTGGCTCTAGAATAACTTCGTATAAAGTATCCTATACGAAGTTATAGGTACCTGAGCTCAACATCGATAGCACTAGTATAACTTCGTATAATGTATGCTATACGAAGTTATGTCGACATCATAATCAACCTCTGGATTACAAAATTTGTGAAAGATTGACTGGTATTCTTAACTATGTTGCTCCTTTTACGCTATGTGGATACGCTGCTTTAATGCCTTTGTATCATGCTATTGCTTCCCGTATGGCTTTCATTTTCTCCTCCTTGTATAAATCCTGGTTAGTTCTTGCCACGGCGGAACTCATCGCCGCCTGCCTTGCCCGCTGCTGGACAGGGGCTCGGCTGTTGGGCACTGACAATTCCGTGGCTCGAGAGATCTTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGAGATCTCACGTGCGGACCGAGCGGCCGCAGGAACCCCTAGTGATGGAGTTGGCCACTCCCTCTCTGCGCGCTCGCTCGCTCACTGAGGCCGGGCGACCAAAGGTCGCCCGACGCCCGGGCTTTGCCCGGGCGGCCTCAGTGAGCGAGCGAGCGCGCAGCTGCCTGCAGGGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATACGTCAAAGCAACCATAGTACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCTTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTTGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACTCTATCTCGGGCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGTCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGGGAATTTTAACAAAATATTAACGTTTACAATTTTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGT  -  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,GDGCHC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,GCCGGC,CAATTG,ACGCGT,TCCRAC,GTYGGA,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,RGCR,YGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                           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                                       	         %         	                  	                   
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                                                    
                         b   a      H         M   M               O                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         &       
                                                        
             	   %   	      	       
           
                  
         b a  H   M M     O   1  2  2  2  6'  9  A*w " 
( p  "+t$%>		
W5 y	fh 	# EDb\# 	## g*7s&			

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d

s'5 y	f5 y	f5 y	fhdjz$*? /	("  	& 		\`Y
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   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  '<?xml version="1.0"?><Features nextValidID="16"><Feature recentID="0" name="AAV2 ITR (alternate)" type="repeat_region" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-130" color="#a6acb3" type="standard"/><Q name="label"><V text="AAV2 ITR (alternate)"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;/Application Notes=Allows replication of the viral genome and its packaging into virus.&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/References=J Virol. 34:402 (1980)&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/color=#5566f5&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="hsA2" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="146-332" color="#ffffff" type="standard"/><Q name="label"><V text="hsA2"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;/color=#ef6cdf&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="eHGT_140h" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="410-713" color="#a6acb3" type="standard"/><Q name="label"><V text="eHGT_140h"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;/color=#c54b7c&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="minRho" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="769-888" color="#ffffff" type="standard"/><Q name="label"><V text="minRho"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;/color=#d05c0a&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="Lox2272" type="Miscellaneous" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="922-955" color="#a6acb3" type="standard"/><Q name="label"><V text="Lox2272"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;/Application Notes=Recognition site of Cre recombinase; can't work with other types of Lox site.&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/Description=Mutated Lox site with two base substitutions of LoxP&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/References=Nucleic Acids Res.30:e103 (2002)&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/color=#fd3434&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="LoxP" type="Miscellaneous" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="988-1021" color="#a6acb3" type="standard"/><Q name="label"><V text="LoxP"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;/Application Notes=Recognition site of Cre recombinase.&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/Description=Locus of X(cross)-over in P1&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/References=Nature. 378:720 (1995)&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/color=#46c6ef&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SYFP2" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1028-1747" color="#a6acb3" type="standard"/><Q name="label"><V text="SYFP2"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;/color=#eda481&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="Kozak sequence" directionality="2" type="regulatory" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1748-1753" color="#ffffff" type="standard"/><Q name="label"><V text="Kozak sequence"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;/Application Notes=Facilitates translation initiation of ATG start codon downstream of the Kozak sequence.&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/Full Name=Kozak&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/References=&amp;lt;a href= &amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;Nucleic Acids Res. 9:5233 (1981)&amp;lt;/a&amp;gt;&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/color=#da3bb5&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="Lox2272" directionality="2" type="Miscellaneous" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1760-1793" color="#a6acb3" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="label"><V text="Lox2272"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;/Application Notes=Recognition site of Cre recombinase; can't work with other types of Lox site.&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/Description=Mutated Lox site with two base substitutions of LoxP&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/color=#e4b930&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="LoxP" directionality="2" type="Miscellaneous" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1826-1859" color="#a6acb3" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="label"><V text="LoxP"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;/Application Notes=Recognition site of Cre recombinase.&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/Description=Locus of X(cross)-over in P1&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/References=Nature. 378:720 (1995)&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/color=#7a00b5&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="WPRE3" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1869-2114" color="#ffffff" type="standard"/><Q name="label"><V text="WPRE3"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;/color=#05696d&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2131-2242" color="#a6acb3" type="standard"/><Q name="label"><V text="bGH poly(A) signal"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;/Application Notes=Allows transcription termination and polyadenylation of mRNA transcribed by Pol II RNA polymerase.&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/Full Name=Bovine growth hormone polyadenylation&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/References=&amp;lt;a href=&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;J Biol Chem. 267:16330 (1992)&amp;lt;/a&amp;gt;; &amp;lt;a href=&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;Nat Protoc. 7:171 (2012)&amp;lt;/a&amp;gt;&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/color=#f54c6b&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="AAV2 ITR" type="repeat_region" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2360-2489" color="#a6acb3" type="standard"/><Q name="label"><V text="AAV2 ITR"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;/Application Notes=Allows rescue of virus from recombinant plasmid and replication of the viral genome; this ITR is identical to that of the wild-type AAV2 genome.&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/References=J Virol. 34:402 (1980)&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/color=#944603&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="f1 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2575-3030" color="#ffff00" type="standard"/><Q name="label"><V text="f1 ori"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;/color=#ce0084&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="AmpR" directionality="1" translationMW="31557.36" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3417-4277" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="label"><V text="AmpR"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;/Application Notes=Allows E. coli to be resistant to ampicillin.&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/Full Name=Ampicillin resistance gene&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/References=Proc Natl Acad Sci U S A. 75: 3737 (1978)&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/Selection Drug=Ampicillin &lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/Subtype=E. coli selection marker&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/Usage=Positive selection&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/color=#6d3ed8&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="15" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4448-5036" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="label"><V text="ori"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;/Application Notes=Facilitates plasmid replication in E. coli; regulates high-copy plasmid number (500-700).&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/Full Name=pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/References=Mol Microbiol. 6:3385 (1992)&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/Subtype=E. coli replication_origin&lt;/body&gt;&lt;/html&gt;"/><V text="&lt;html&gt;&lt;body&gt;/color=#0375bb&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/></Primers>
  T<Notes>
<UUID>266fe2b7-4c0e-4893-bbe6-066ddec2e31e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="22:0:0">2026.7.20</Created>
<LastModified UTC="22:0:0">2026.7.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<Comments>Vector Name: pAAV-hsA2-eHGT140h-minRho_Lox2272/LoxP/rev(SYFP2)/Lox2272/LoxP_WPRE3-bGH(pA)&lt;br>Vector Type: Mammalian Gene Expression AAV Vector&lt;br>Plasmid Copy Number:&amp;nbsp;&amp;nbsp;High&lt;br>Antibiotic Resistance:&amp;nbsp;&amp;nbsp;Ampicillin</Comments>
</Notes>
  Y      K         Unique 6+ Cutters                                                                                            d     THO               E              Y? P                                                                                                                                                           #   <7zXZ  ִF !   t/ []   S.( .~}    YZ   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
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