	   SnapGene      tagccatgctctaggaagatctctgcagagggccctgcgtatgagtgcaagtgggttttaggaccaggatgaggcggggtgggggtgcctacctgacgaccgaccccgacccactggacaagcacccaacccccattccccaaattgcgcatcccctatcagagagggggaggggaaacaggatgcggcgaggcgcgtgcgcactgccagcttcagcaccgcggacagtgccttcgcccccgcctggcggcgcgcgccaccgccgcctcagcactgaaggcgcgctgacgtcactcgccggtcccccgcaaactccccttcccggccaccttggtcgcgtccgcgccgccgccggcccagccggaccgcaccacgcgaggcgcgagataggggggcacgggcgcgaccatctgcgctgcggcgccggcgactcagcgctgcctcagtctgcggtgggcagcggaggagtcgtgtcgtgcctgagagcgcagtcgaattcaagctgctagcaaggatccccataacttcgtataaagtatcctatacgaagttatatcaaaataggaagaccaatgcttcaccatcgacccgaattgccaagcatcaccatcgacccataacttcgtataatgtatgctatacgaagttatactagagctttcacttcgctgtcatcatttgtacaaactcttcgtagtttacctgaccatccccatcgatgtctgcttccctgatcatttcatcaacctcttcatctgttaacttctctccaaggtttgtcatcacgtggcgaagctctgctgcactgatgtagccattgccatccttatcaaacacaccgaacgcttctctaatttcttcttccgtgtccctgtatttcatttttcttgccatcattgtcaggaactcagggaagtcgattgtgccgtcaccgtcggcatctacttcattgatcatgtcctgcagctctgcttctgtggggttctgccccagagaccgcatcaccgtccccagctccttggttgttattgtcccatccccgtccttgtcaaatagggagaaagcctctttgtattctgcgatctgctcttcagtcagttggtccggcaggttgtactccagcttgtgccccaggatgttgccgtcctccttgaagtcgatgcccttcagctcgatgcggttcaccagggtgtcgccctcgaacttcacctcggcgcgggtcttgtagttgccgtcgtccttgaagaagatggtgcgctcctggatgtagccttcgggcatggcggacttgaagaagtcgtgctgcttcatgtggtcggggtagcggctgaagcactgcacgccgtaggtcagggtggtcacgagggtgggccagggcacgggcagcttgccggtggtgcagatgaacttcagggtcagcttgccgtaggtggcatcgccctcaccctcgccggacacgctgaacttgtggccgtttacgtcgccgtccagctcgaccaggatgggcaccaccccggtgaacagctcctcgcccttgctcaccatgctccctccggtaccgcccttgtacagctcgtccatgccgagagtgatcccggcggcggtcacgaactccagcaggaccatgtgatcgcgcttctcgttggggtctttcgaaagtttggactccacgctcaggtagtggttgtcgggcagcagcacggggccgtcgccgatgggggtgttctgctggtagtggtaggcgagctgcacgccgccgtcctcgatgttgtggcggatgtggaagttcgccttgatgccgttcttctgcttgtcggccttgatatagacgttgatcttcaggcccatcaggcggccgatgatcttcacggcggtggccacctccttgaaggtcttcttgcgacgcgtgtgatgatggtgatgatgcatggtggcggatcctagtataacttcgtataggatactttatacgaagttatcattgggattcttcctattttgatccaagcatcaccatcgaccctctagtccagatctcaccatcgacccataacttcgtatagcatacattatacgaagttatgtccctcgaagaggttcgaattcgatatcaagcttatcgataatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcatcgataccgtcgacccgggcggccgcttcgagcagacatgataagatacattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggagatgtgggaggttttttaaagcaagtaaaacctctacaaatgtggtaaaatcgataaggatcttcctagagcatggctacgtagataagtagcatggcgggttaatcattaactacaaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttccttgtagttaatgattaacccgccatgctacttatctacg  0n  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,GDGCHC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,GCCGGC,CAATTG,ACGCGT,TCCRAC,GTYGGA,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,RGCR,YGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                    $                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               	                                                                     	                                                                                                                                                                                     (                                                       	                                              
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s#   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  !-<?xml version="1.0"?><Features nextValidID="15"><Feature recentID="0" name="lox2272" directionality="2" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1946-1979" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;Cre recombinase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Cre-mediated recombination occurs in the 8-bp core sequence (AAGTATCC) (&lt;u&gt;&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116776/&quot;&gt;Shaw et al., 2021&lt;/u&gt;&lt;/a&gt;). &lt;i&gt;lox2272&lt;/i&gt; sites are compatible with each other, but incompatible with &lt;i&gt;loxP&lt;/i&gt; or &lt;i&gt;loxN&lt;/i&gt; sites (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/9714735&quot;&gt;Lee and Saito, 1988&lt;/a&gt;).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="lox2272" directionality="1" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="521-554" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;Cre recombinase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Cre-mediated recombination occurs in the 8-bp core sequence (AAGTATCC) (&lt;u&gt;&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116776/&quot;&gt;Shaw et al., 2021&lt;/u&gt;&lt;/a&gt;). &lt;i&gt;lox2272&lt;/i&gt; sites are compatible with each other, but incompatible with &lt;i&gt;loxP&lt;/i&gt; or &lt;i&gt;loxN&lt;/i&gt; sites (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/9714735&quot;&gt;Lee and Saito, 1988&lt;/a&gt;).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="loxP" directionality="2" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2050-2083" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;Cre recombinase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Cre-mediated recombination occurs in the 8-bp core sequence (ATGTATGC) (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116776/&quot;&gt;Shaw et al., 2021&lt;/a&gt;).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="loxP" directionality="1" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="617-650" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;Cre recombinase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Cre-mediated recombination occurs in the 8-bp core sequence (ATGTATGC) (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116776/&quot;&gt;Shaw et al., 2021&lt;/a&gt;).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="Factor Xa site" directionality="2" translationMW="473.53" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="2595" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="2521" maxFusedRunOn="2521" detectionMode="exactProteinMatch"><Segment range="2596-2607" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IEGR"/></Q></Feature><Feature recentID="5" name="6xHis" directionality="2" translationMW="840.86" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="661" maxFusedRunOn="661" detectionMode="exactProteinMatch"><Segment range="1909-1926" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;6xHis affinity tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="HHHHHH"/></Q></Feature><Feature recentID="6" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2125-2713" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="SV40 poly(A) signal" type="polyA_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2756-2877" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="hSyn promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="44-491" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human synapsin I promoter; confers neuron-specific expression (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/12595892&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/12595892&quot;&gt;Kügler et al., 2003&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5155-5610" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3405-3993" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4955" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4164-4955" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="4956-5024" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="12" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5025-5129" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="AAV2 ITR" type="repeat_region" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" prioritize="1" originalSequence="CTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTAGGGGTTCCT"><Segment range="5682-5811" color="#ffcc99" type="standard"/></Feature><Feature recentID="14" name="AAV2 ITR" type="repeat_region" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" prioritize="1" originalSequence="CTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTAGGGGTTCCT"><Segment range="3039-3168" color="#ffcc99" type="standard"/></Feature></Features>
  V<?xml version="1.0"?><Primers nextValidID="10"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="Amp-R" sequence="ATAATACCGCGCCACATAGC" description="Ampicillin resistance gene, reverse primer"><BindingSite location="4786-4805" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="4786-4805" bases="ATAATACCGCGCCACATAGC"/></BindingSite><BindingSite simplified="1" location="4786-4805" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="4786-4805" bases="ATAATACCGCGCCACATAGC"/></BindingSite></Primer><Primer recentID="1" name="EBV-rev" sequence="GTGGTTTGTCCAAACTCATC" description="SV40 polyA terminator, reverse primer"><BindingSite location="2766-2785" boundStrand="1" annealedBases="GTGGTTTGTCCAAACTCATC" meltingTemperature="55"><Component hybridizedRange="2766-2785" bases="GTGGTTTGTCCAAACTCATC"/></BindingSite><BindingSite simplified="1" location="2766-2785" boundStrand="1" annealedBases="GTGGTTTGTCCAAACTCATC" meltingTemperature="55"><Component hybridizedRange="2766-2785" bases="GTGGTTTGTCCAAACTCATC"/></BindingSite></Primer><Primer recentID="2" name="EGFP-C" sequence="CATGGTCCTGCTGGAGTTCGTG" description="EGFP, forward primer"><BindingSite location="1605-1626" boundStrand="1" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="1605-1626" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite><BindingSite simplified="1" location="1605-1626" boundStrand="1" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="1605-1626" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite></Primer><Primer recentID="3" name="EGFP-N" sequence="CGTCGCCGTCCAGCTCGACCAG" description="EGFP, reverse primer"><BindingSite location="1478-1499" boundStrand="0" annealedBases="CGTCGCCGTCCAGCTCGACCAG" meltingTemperature="67"><Component hybridizedRange="1478-1499" bases="CGTCGCCGTCCAGCTCGACCAG"/></BindingSite><BindingSite simplified="1" location="1478-1499" boundStrand="0" annealedBases="CGTCGCCGTCCAGCTCGACCAG" meltingTemperature="67"><Component hybridizedRange="1478-1499" bases="CGTCGCCGTCCAGCTCGACCAG"/></BindingSite></Primer><Primer recentID="4" name="EXFP-R" sequence="GTCTTGTAGTTGCCGTCGTC" description="For distinguishing EGFP variants, reverse primer"><BindingSite location="1219-1238" boundStrand="0" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="1219-1238" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite><BindingSite simplified="1" location="1219-1238" boundStrand="0" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="1219-1238" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite></Primer><Primer recentID="5" name="F1ori-F" sequence="GTGGACTCTTGTTCCAAACTGG" description="F1 origin, forward primer"><BindingSite location="3642-3651" boundStrand="0" annealedBases="TCCAACTGG" meltingTemperature="13"><Component bases="GTGGACTCTTGT"/><Component hybridizedRange="3642-3646" bases="TCCAA"/><Component bases="A"/><Component hybridizedRange="3648-3651" bases="CTGG"/></BindingSite><BindingSite location="5291-5312" boundStrand="1" annealedBases="GTGGACTCTTGTTCCAAACTGG" meltingTemperature="57"><Component hybridizedRange="5291-5312" bases="GTGGACTCTTGTTCCAAACTGG"/></BindingSite><BindingSite simplified="1" location="3642-3651" boundStrand="0" annealedBases="TCCAACTGG" meltingTemperature="13"><Component bases="GTGGACTCTTGT"/><Component hybridizedRange="3642-3646" bases="TCCAA"/><Component bases="A"/><Component hybridizedRange="3648-3651" bases="CTGG"/></BindingSite><BindingSite simplified="1" location="5291-5312" boundStrand="1" annealedBases="GTGGACTCTTGTTCCAAACTGG" meltingTemperature="57"><Component hybridizedRange="5291-5312" bases="GTGGACTCTTGTTCCAAACTGG"/></BindingSite></Primer><Primer recentID="6" name="F1ori-R" sequence="AGGGAAGAAAGCGAAAGGAG" description="F1 origin, reverse primer"><BindingSite location="5503-5522" boundStrand="0" annealedBases="AGGGAAGAAAGCGAAAGGAG" meltingTemperature="57"><Component hybridizedRange="5503-5522" bases="AGGGAAGAAAGCGAAAGGAG"/></BindingSite><BindingSite simplified="1" location="5503-5522" boundStrand="0" annealedBases="AGGGAAGAAAGCGAAAGGAG" meltingTemperature="57"><Component hybridizedRange="5503-5522" bases="AGGGAAGAAAGCGAAAGGAG"/></BindingSite></Primer><Primer recentID="7" name="pBR322ori-F" sequence="GGGAAACGCCTGGTATCTTT" description="pBR322 origin, forward primer"><BindingSite location="3484-3503" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="3484-3503" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite><BindingSite simplified="1" location="3484-3503" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="3484-3503" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite></Primer><Primer recentID="8" name="SV40pA-R" sequence="GAAATTTGTGATGCTATTGC" description="SV40 polyA, reverse primer"><BindingSite location="2227-2236" boundStrand="0" annealedBases="ATGCTATTGC" meltingTemperature="32"><Component bases="GAAATTTGTG"/><Component hybridizedRange="2227-2236" bases="ATGCTATTGC"/></BindingSite><BindingSite location="2820-2839" boundStrand="0" annealedBases="GAAATTTGTGATGCTATTGC" meltingTemperature="52"><Component hybridizedRange="2820-2839" bases="GAAATTTGTGATGCTATTGC"/></BindingSite><BindingSite simplified="1" location="2227-2236" boundStrand="0" annealedBases="ATGCTATTGC" meltingTemperature="32"><Component bases="GAAATTTGTG"/><Component hybridizedRange="2227-2236" bases="ATGCTATTGC"/></BindingSite><BindingSite simplified="1" location="2820-2839" boundStrand="0" annealedBases="GAAATTTGTGATGCTATTGC" meltingTemperature="52"><Component hybridizedRange="2820-2839" bases="GAAATTTGTGATGCTATTGC"/></BindingSite></Primer><Primer recentID="9" name="WPRE-R" sequence="CATAGCGTAAAAGGAGCAACA" description="WPRE, reverse primer"><BindingSite location="2177-2197" boundStrand="1" annealedBases="CATAGCGTAAAAGGAGCAACA" meltingTemperature="55"><Component hybridizedRange="2177-2197" bases="CATAGCGTAAAAGGAGCAACA"/></BindingSite><BindingSite simplified="1" location="2177-2197" boundStrand="1" annealedBases="CATAGCGTAAAAGGAGCAACA" meltingTemperature="55"><Component hybridizedRange="2177-2197" bases="CATAGCGTAAAAGGAGCAACA"/></BindingSite></Primer></Primers>
  B<Notes>
<UUID>503f3cb2-4cdd-497f-98f0-048ba4888d86</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pGP-AAV-syn-FLEX-jGCaMP8m-WPRE</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression.</Description>
<Created UTC="5:0:0">2025.12.15</Created>
<LastModified UTC="5:0:0">2025.12.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<References>
<Reference authors="Zhang Y, Rózsa M, Bushey D, Zheng J, Reep D, Broussard GJ, Tsang A, Tsegaye G, Patel R, Narayan S, Li JX, Zhang R, Ahrens MB, Turner GC, Wang SS-H, Svoboda K, Korff W, Schreiter ER, Hasseman JP, Kolb I, Looger LL" journal="Janelia Research Campus, Online Resource (2020) doi:10.25378/janelia.13148243." journalName="Janelia Research Campus, Online Resource (2020) doi:10.25378/janelia.13148243." title="jGCaMP8 Fast Genetically Encoded Calcium Indicators" type="Journal Article"/>
</References>
</Notes>
  Y              Unique 6+ Cutters                                                                                            d     THO  d              E              Y? P                                                                                                                                                           #   <7zXZ  ִF !   t/ []   S.( .~}    YZ   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
