	   SnapGene      cgccggcgactcagcgctgcctcagtctgcggtgggcagcggaggagtcgtgtcgtgcctgagagcgcagtcgaattcaagctgctagcaaggatccacccgccaccatgctgcagaacgagcttgctcttaagttggctggacttgatattaacaagactggaggaggttctcatcatcatcatcatcatggtatggctagcatgactggtggacagcaaatgggtcgggatctgtacgacgatgacgataaggatctcgcaacaatggtcgactcatcacgtcgtaagtggaataagacaggtcacgcagtcagagctataggtcggctgagctcagcgatcaactgcgaaatgatgtacccagcggatggtggtctgcgtggttacactcacatggcgctgaaagttgatggcggcggtcacctgtcctgttctttcgtgaccacctaccgctccaaaaagactgtcggcaacattaagatgcctgccattcatagcgtcagccaccgtctggagcgcctggaggagagcgataacgaaatgtttgtcgtacagcgtgaacacgcagttgccaagtttgtgggcctgggtggtggcggcggtaccggagggagcatgaactccctgatcaaggagaacatgcgtatgaaagtggttctggaaggctccgtaaacggccaccagttcaaatgcactggtgaaggcgaaggcaacccgtatatgggcacccagactatgcgtatcaaagtgatcgagggtggtccgctgccgtttgcgttcgacatcctggcgacgtcctttatgtatggctcccgtaccttcatcaaatatccgaaaggcatcccggatttctttaagcagtccttcccggaaggttttacctgggaacgtgtgacccgttacgaagacggcggcgtaattaccgttatgcaagacacgtctctggaggatggctgcctggtgtatcacgtgcaggttcgcggtgtgaacttcccgagcaatggtgctgtaatgcaaaagaaaaccaaaggttgggagcctacggactcccaaaccactgaagagcagatcgcagaatttaaagaggctttctccctatttgacaaggacggggatgggacaataacaaccaaggagctggggacggtgatgcggtctctggggcagaaccccacagaagcagagctgcaggacatgatcaatgaagtagatgccgacggtgacggcacaatcgacttccctgagttcctgattatgatggcaagaaaaatgaaatacacagacagtgaagaagaaattagagaagcgttcggcgtgtttgataaggatggcaatggctacatcagtgcagcagagcttcgccacgtgatgacaaaccttggagagaagttaacagatgaagaggttgatgaaatgatcagggaagcagacagcgatggggatggtcaggtaaactacgaagagtttgtacaaatgatgacagcgaagtgaaagcttatcgataatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcatcgataccgtcgacctcgacccgggcggccgcttcgagcagacatgataagatacattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggagatgtgggaggttttttaaagcaagtaaaacctctacaaatgtggtaaaatcgataaggatcttcctagagcatggctacgtagataagtagcatggcgggttaatcattaactacaaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagcgcgcagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgcgtcccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctgcgcgctcgctcgctcactgaggccgcccgggcaaagcccgggcgtcgggcgacctttggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcactaggggttccttgtagttaatgattaacccgccatgctacttatctacgtagccatgctctaggaagatctctgcagagggccctgcgtatgagtgcaagtgggttttaggaccaggatgaggcggggtgggggtgcctacctgacgaccgaccccgacccactggacaagcacccaacccccattccccaaattgcgcatcccctatcagagagggggaggggaaacaggatgcggcgaggcgcgtgcgcactgccagcttcagcaccgcggacagtgccttcgcccccgcctggcggcgcgcgccaccgccgcctcagcactgaaggcgcgctgacgtcactcgccggtcccccgcaaactccccttcccggccaccttggtcgcgtccgcgccgccgccggcccagccggaccgcaccacgcgaggcgcgagataggggggcacgggcgcgaccatctgcgctgcgg  -  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          &                                                                                                                                                                                                          
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   	         	                           &   $         -                  V   7         L   R               M                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               &                                                                        
   4    	    
      
       
   	    
 	   	         & $   -      V 7   L R     M   4	  4  5  5C  8  =g  Cjjjq/t<		


r/u<`c	"cESz+0 
	= 8	 8	e( 
qp 4%)	$
g

$?ik<Pjsy=kY~9	"l	"	" %
]c( GG8     		}HM}/
%4>	Kc79ZZe
n+}H		
3H 4#FT	?  y
KmB			S  K	3[ K>.5	W,{rsTn >rx ,
08[ Z)z	

QzMG  	|/jTQ	pJrkbYyB		JN]xJ4] w		#mo)  gHirCHS
+K>Kmzj
kby0
<%x S y  %.						c


-;#6AmoYNn
Q	p[   =	+6=B		N7	f
K  	#|FFB3H n7 .n7Vg {0z}jh m $ k0	NAn 	7 /I,-R
Xt ~8M	[   =	+\ 7iK }/FP|Jrx
>Kfgj # P m E22~		K
]
 ADZPGQLdBC					%<{u# E	AD P m 22~	K
]
 ZPK<
p[0	 # P m UE2"2~!GJ			K
]
`

q ADZ'Pft,jyJLdrxBC					
>K%<fH,~)  =JUFoZ/(I		  N	V~: I	^vy4

T	  : IN		V~A	XVU!N	B
<I=	R@b /)c^bd	
- , 
F

"Tj 6	)08@hO3`Zh*
R{Hdo*/}N(bKW%	x4@	1
=, T F
"j/}(K

U"!GJ	`

q'ft,jy!AN		B
<IXV=Uiv
5_@&X		


gTTh$5Onpc #0"*K5


rfSdgu_Ncwz & mUXGd		

`
g
|
l'p-H,Pj f'[0O x <LqL	o
QGD @ 				
-
N
\U19Sir#%4:moqT  E		4B~\   Tt->	,7?WiH I			




Q4 ^MeC			y&xK=j   hGYK	`	
{fpkcxO x   <GLH)?q9L[?D} IN						

\
o




QFEQz=G4uDj    ?h"&'GXqYK	`	

4e{:fp$[8kwcxyUR	i	~eq
qo D	\w 9 jJ^qMerx&C			


>Kcny&Ux~K=FBf    , 	K^Messy6;C			



.?Ly&>KROxK=]<{&gI 5U i%<"			;
V})   a={		
aB,1  W8o8	i	p	 
lg^mSZO x  -<BL2jqyqLUa|;	!	R			o
Qbi	G~eq
qDF /8>P6	   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  "<?xml version="1.0"?><Features recycledIDs="17,16" nextValidID="18"><Feature recentID="0" name="AAV2 ITR" type="repeat_region" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" prioritize="1" originalSequence="CTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTAGGGGTTCCT"><Segment range="5069-5198" color="#ffcc99" type="standard"/></Feature><Feature recentID="1" name="AAV2 ITR" type="repeat_region" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" prioritize="1" originalSequence="CTGCGCGCTCGCTCGCTCACTGAGGCCGCCCGGGCAAAGCCCGGGCGTCGGGCGACCTTTGGTCGCCCGGCCTCAGTGAGCGAGCGAGCGCGCAGAGAGGGAGTGGCCAACTCCATCACTAGGGGTTCCT"><Segment range="2426-2555" color="#ffcc99" type="standard"/></Feature><Feature recentID="2" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4412-4516" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="4342" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="3551-4342" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="4343-4411" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="4" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2792-3380" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="f1 ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4542-4997" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="calmodulin-binding peptide" directionality="1" translationMW="2193.55" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1493" maxFusedRunOn="1493" detectionMode="exactProteinMatch"><Segment range="282-338" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;calmodulin-binding peptide from avian smooth muscle myosin light chain kinase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="RRKWNKTGHAVRAIGRLSS"/></Q></Feature><Feature recentID="7" name="hSyn promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5280-70" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human synapsin I promoter; confers neuron-specific expression (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/12595892&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/12595892&quot;&gt;Kügler et al., 2003&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="SV40 poly(A) signal" type="polyA_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2143-2264" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1506-2094" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="T7 tag (gene 10 leader)" directionality="1" translationMW="1098.29" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1493" maxFusedRunOn="1493"><Segment range="195-227" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promotes efficient translation in &lt;i&gt;E. coli&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;leader peptide from bacteriophage T7 gene &lt;i&gt;10&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MASMTGGQQMG"/></Q></Feature><Feature recentID="11" name="6xHis" directionality="1" translationMW="840.86" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1493" maxFusedRunOn="1493" detectionMode="exactProteinMatch"><Segment range="174-191" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;6xHis affinity tag&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="HHHHHH"/></Q></Feature><Feature recentID="12" name="Xpress™ tag" directionality="1" translationMW="997.97" type="CDS" allowSegmentOverlaps="0" cleavageArrows="254" consecutiveTranslationNumbering="1" maxRunOn="1493" maxFusedRunOn="1493" detectionMode="exactProteinMatch"><Segment range="231-239" color="#cc99b2" type="standard" translated="1"/><Segment name="enterokinase site" range="240-254" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Xpress™ epitope tag, including an enterokinase recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="DLY,DDDDK"/></Q></Feature><Feature recentID="13" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="1976" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="1902" maxFusedRunOn="1902" detectionMode="exactProteinMatch"><Segment range="1977-1988" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IEGR"/></Q></Feature><Feature recentID="14" name="KS primer" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2097-2113" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="jRCaMP1a" directionality="1" translationMW="51029.58" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="108-1493" color="#993366" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MLQNELALKLAGLDINKTGGGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNKTGHAVRAIGRLSSAINCEMMYPADGGLRGYTHMALKVDGGGHLSCSFVTTYRSKKTVGNIKMPAIHSVSHRLERLEESDNEMFVVQREHAVAKFVGLGGGGGTGGSMNSLIKENMRMKVVLEGSVNGHQFKCTGEGEGNPYMGTQTMRIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVITVMQDTSLEDGCLVYHVQVRGVNFPSNGAVMQKKTKGWEPTDSQTTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGDGTIDFPEFLIMMARKMKYTDSEEEIREAFGVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADSDGDGQVNYEEFVQMMTAK*"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="9"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="0" name="Amp-R" sequence="ATAATACCGCGCCACATAGC" description="Ampicillin resistance gene, reverse primer" visible="0"><BindingSite location="4173-4192" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="4173-4192" bases="ATAATACCGCGCCACATAGC"/></BindingSite><BindingSite simplified="1" location="4173-4192" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="4173-4192" bases="ATAATACCGCGCCACATAGC"/></BindingSite></Primer><Primer recentID="1" name="EBV-rev" sequence="GTGGTTTGTCCAAACTCATC" description="SV40 polyA terminator, reverse primer" visible="0"><BindingSite location="2153-2172" boundStrand="1" annealedBases="GTGGTTTGTCCAAACTCATC" meltingTemperature="55"><Component hybridizedRange="2153-2172" bases="GTGGTTTGTCCAAACTCATC"/></BindingSite><BindingSite simplified="1" location="2153-2172" boundStrand="1" annealedBases="GTGGTTTGTCCAAACTCATC" meltingTemperature="55"><Component hybridizedRange="2153-2172" bases="GTGGTTTGTCCAAACTCATC"/></BindingSite></Primer><Primer recentID="2" name="F1ori-F" sequence="GTGGACTCTTGTTCCAAACTGG" description="F1 origin, forward primer" visible="0"><BindingSite location="3029-3038" boundStrand="0" annealedBases="TCCAACTGG" meltingTemperature="13"><Component bases="GTGGACTCTTGT"/><Component hybridizedRange="3029-3033" bases="TCCAA"/><Component bases="A"/><Component hybridizedRange="3035-3038" bases="CTGG"/></BindingSite><BindingSite location="4678-4699" boundStrand="1" annealedBases="GTGGACTCTTGTTCCAAACTGG" meltingTemperature="57"><Component hybridizedRange="4678-4699" bases="GTGGACTCTTGTTCCAAACTGG"/></BindingSite><BindingSite simplified="1" location="3029-3038" boundStrand="0" annealedBases="TCCAACTGG" meltingTemperature="13"><Component bases="GTGGACTCTTGT"/><Component hybridizedRange="3029-3033" bases="TCCAA"/><Component bases="A"/><Component hybridizedRange="3035-3038" bases="CTGG"/></BindingSite><BindingSite simplified="1" location="4678-4699" boundStrand="1" annealedBases="GTGGACTCTTGTTCCAAACTGG" meltingTemperature="57"><Component hybridizedRange="4678-4699" bases="GTGGACTCTTGTTCCAAACTGG"/></BindingSite></Primer><Primer recentID="3" name="F1ori-R" sequence="AGGGAAGAAAGCGAAAGGAG" description="F1 origin, reverse primer" visible="0"><BindingSite location="4890-4909" boundStrand="0" annealedBases="AGGGAAGAAAGCGAAAGGAG" meltingTemperature="57"><Component hybridizedRange="4890-4909" bases="AGGGAAGAAAGCGAAAGGAG"/></BindingSite><BindingSite simplified="1" location="4890-4909" boundStrand="0" annealedBases="AGGGAAGAAAGCGAAAGGAG" meltingTemperature="57"><Component hybridizedRange="4890-4909" bases="AGGGAAGAAAGCGAAAGGAG"/></BindingSite></Primer><Primer recentID="4" name="L4440" sequence="AGCGAGTCAGTGAGCGAG" description="L4440 vector, forward primer" visible="0"><BindingSite location="748-757" boundStrand="0" annealedBases="AGTGACGAG" meltingTemperature="21"><Component bases="AGCGAGTC"/><Component hybridizedRange="748-752" bases="AGTGA"/><Component bases="G"/><Component hybridizedRange="754-757" bases="CGAG"/></BindingSite><BindingSite location="2471-2482" boundStrand="1" annealedBases="TCAGTGAGCGAG" meltingTemperature="42"><Component bases="AGCGAG"/><Component hybridizedRange="2471-2482" bases="TCAGTGAGCGAG"/></BindingSite><BindingSite location="2526-2543" boundStrand="0" annealedBases="GCGTCAGTGAGCGAG" meltingTemperature="42"><Component bases="A"/><Component hybridizedRange="2526-2528" bases="GCG"/><Component bases="AG"/><Component hybridizedRange="2532-2543" bases="TCAGTGAGCGAG"/></BindingSite><BindingSite location="2620-2637" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="2620-2637" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite><BindingSite location="5079-5096" boundStrand="1" annealedBases="GCGTCAGTGAGCGAG" meltingTemperature="42"><Component bases="A"/><Component hybridizedRange="5094-5096" bases="GCG"/><Component bases="AG"/><Component hybridizedRange="5079-5090" bases="TCAGTGAGCGAG"/></BindingSite><BindingSite location="5140-5151" boundStrand="0" annealedBases="TCAGTGAGCGAG" meltingTemperature="42"><Component bases="AGCGAG"/><Component hybridizedRange="5140-5151" bases="TCAGTGAGCGAG"/></BindingSite><BindingSite simplified="1" location="748-757" boundStrand="0" annealedBases="AGTGACGAG" meltingTemperature="21"><Component bases="AGCGAGTC"/><Component hybridizedRange="748-752" bases="AGTGA"/><Component bases="G"/><Component hybridizedRange="754-757" bases="CGAG"/></BindingSite><BindingSite simplified="1" location="2471-2482" boundStrand="1" annealedBases="TCAGTGAGCGAG" meltingTemperature="42"><Component bases="AGCGAG"/><Component hybridizedRange="2471-2482" bases="TCAGTGAGCGAG"/></BindingSite><BindingSite simplified="1" location="2532-2543" boundStrand="0" annealedBases="TCAGTGAGCGAG" meltingTemperature="42"><Component bases="AGCGAG"/><Component hybridizedRange="2532-2543" bases="TCAGTGAGCGAG"/></BindingSite><BindingSite simplified="1" location="2620-2637" boundStrand="1" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="2620-2637" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite><BindingSite simplified="1" location="5079-5090" boundStrand="1" annealedBases="TCAGTGAGCGAG" meltingTemperature="42"><Component bases="AGCGAG"/><Component hybridizedRange="5079-5090" bases="TCAGTGAGCGAG"/></BindingSite><BindingSite simplified="1" location="5140-5151" boundStrand="0" annealedBases="TCAGTGAGCGAG" meltingTemperature="42"><Component bases="AGCGAG"/><Component hybridizedRange="5140-5151" bases="TCAGTGAGCGAG"/></BindingSite></Primer><Primer recentID="5" name="pBluescriptKS" sequence="TCGAGGTCGACGGTATC" description="For pBluescript vector" visible="0"><BindingSite location="2097-2113" boundStrand="1" annealedBases="TCGAGGTCGACGGTATC" meltingTemperature="54"><Component hybridizedRange="2097-2113" bases="TCGAGGTCGACGGTATC"/></BindingSite><BindingSite simplified="1" location="2097-2113" boundStrand="1" annealedBases="TCGAGGTCGACGGTATC" meltingTemperature="54"><Component hybridizedRange="2097-2113" bases="TCGAGGTCGACGGTATC"/></BindingSite></Primer><Primer recentID="6" name="pBR322ori-F" sequence="GGGAAACGCCTGGTATCTTT" description="pBR322 origin, forward primer" visible="0"><BindingSite location="2871-2890" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="2871-2890" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite><BindingSite simplified="1" location="2871-2890" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="2871-2890" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite></Primer><Primer recentID="7" name="SV40pA-R" sequence="GAAATTTGTGATGCTATTGC" description="SV40 polyA, reverse primer" visible="0"><BindingSite location="1608-1617" boundStrand="0" annealedBases="ATGCTATTGC" meltingTemperature="32"><Component bases="GAAATTTGTG"/><Component hybridizedRange="1608-1617" bases="ATGCTATTGC"/></BindingSite><BindingSite location="2207-2226" boundStrand="0" annealedBases="GAAATTTGTGATGCTATTGC" meltingTemperature="52"><Component hybridizedRange="2207-2226" bases="GAAATTTGTGATGCTATTGC"/></BindingSite><BindingSite simplified="1" location="1608-1617" boundStrand="0" annealedBases="ATGCTATTGC" meltingTemperature="32"><Component bases="GAAATTTGTG"/><Component hybridizedRange="1608-1617" bases="ATGCTATTGC"/></BindingSite><BindingSite simplified="1" location="2207-2226" boundStrand="0" annealedBases="GAAATTTGTGATGCTATTGC" meltingTemperature="52"><Component hybridizedRange="2207-2226" bases="GAAATTTGTGATGCTATTGC"/></BindingSite></Primer><Primer recentID="8" name="WPRE-R" sequence="CATAGCGTAAAAGGAGCAACA" description="WPRE, reverse primer" visible="0"><BindingSite location="1558-1578" boundStrand="1" annealedBases="CATAGCGTAAAAGGAGCAACA" meltingTemperature="55"><Component hybridizedRange="1558-1578" bases="CATAGCGTAAAAGGAGCAACA"/></BindingSite><BindingSite simplified="1" location="1558-1578" boundStrand="1" annealedBases="CATAGCGTAAAAGGAGCAACA" meltingTemperature="55"><Component hybridizedRange="1558-1578" bases="CATAGCGTAAAAGGAGCAACA"/></BindingSite></Primer></Primers>
  .<Notes>
<UUID>0715700c-421b-4a48-9b8e-37d326b96aa1</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>AAV_hSyn_NES-jRCaMP1a_WPRE-SV40(pA)</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>AAV expression of jRCaMP1a, a red fluorescent calcium sensor protein, from the Synapsin promoter.</Description>
<Created UTC="20:9:26">2018.8.6</Created>
<LastModified UTC="10:52:14">2024.11.12</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<References>
<Reference title="Sensitive red protein calcium indicators for imaging neural activity." pubMedID="27011354" journal="Elife. 2016 Mar 24;5. pii: e12727. doi: 10.7554/eLife.12727." authors="Dana H, Mohar B, Sun Y, Narayan S, Gordus A, Hasseman JP, Tsegaye G, Holt GT, Hu A, Walpita D, Patel R, Macklin JJ, Bargmann CI, Ahrens MB, Schreiter ER, Jayaraman V, Looger LL, Svoboda K, Kim DS" journalName="Elife. 2016 Mar 24;5. pii: e12727. doi: 10.7554/eLife.12727." type="Journal Article"/>
</References>
</Notes>
  Y              Unsaved Enzyme Set                                                                                           d     CHO  d              E              Y? P                                                                                                                                                              3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
  a<?xml version="1.0"?><CustomEnzymeSets><CustomEnzymeSet type="2" name="Unsaved Enzyme Set" enzymeNames="Acc65I AfeI AflII AlwNI ApaI BamHI BbvCI BfuAI BglII BmrI Bpu10I BsmBI BspMI BsrGI BstEII DraIII EagI Eco53kI EcoNI EcoRI Esp3I FspAI HindIII KasI KpnI MfeI MreI NarI NotI PciI PluTI PspOMI RsrII SacI SalI ScaI SfoI SgrAI XmnI"/></CustomEnzymeSets>
